Effects of all-trans retinoic acid(ATRA) on differentiation and gene expression have been investigated in human choriocarcinoma cell line JEG-3. RA, an active metabolite of vitamine A, has antitumor effects through induction of differentiation,
inhibition of angiogenesis, inhibition of cell proliferation, and suppression of viral oncogenes. ATRA is one of important mediators of cellular differentitation and has been shown to stimulate human chorionic gonadotropin(hCG) secretion by JEG-3
choriocarcinoma cells in vitro. In contrast to proliferation of JEG-3 cells was inhibited by Methotrexate, ATRA did not significantly alter cell numbers in JEG-3 cell lines. β-hCG secretion by JEG-3 cells was stimulated at high concentration of
ATRA
10E-6M. The diffeerential display technique was applied to compare mRNAs from JEG-3 cells and retinoic acid stimulated JEG-3 cells. Complementary DNA fragments corresponding to several apparently diffentially expressed nRNAs were recovered and
sequenced. In conclusion, ARTA physiologic concentration stimulates the differentiation of JEG-3 cells without increasing the cell number and induced and suppreses the expression of some genes.
Trophoblast, JEG-3 cell, all-trans retinoic acid, differentiation.
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